DNA

Part:BBa_K4373001:Design

Designed by: Mandy Hobusch   Group: iGEM22_Lund   (2022-09-24)


Glyphosate dehydrogenase goxB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 760
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The orginial sequence was not codon optimized for Bacillus subtilis. We performed codon optimization for our expression host.


Source

The sequence was obtained from NCBI gene bank [3].

References

[1] Lopes Catão, A. J., & López-Castillo, A. (2018). On the degradation pathway of glyphosate and glycine. Environmental Science: Processes & Impacts, 20(8), 1148–1157. https://doi.org/10.1039/c8em00119g

[2] Quinn, J. P., & Dick, J. E. (1994, October 24). Glyphosate-degrading isolates from environmental samples: occurrence and pathways of degradatio. Applied Microbiology Biotechnology, 43, 545–550.

[3] U.S. National Library of Medicine. (n.d.). Uncultured bacterium clone pgoxb fad-dependent glyphosate oxidase (GOX - nucleotide - NCBI. National Center for Biotechnology Information. Retrieved September 24, 2022, from https://www.ncbi.nlm.nih.gov/nucleotide/GU479463.1?report=genbank&log%24=nucltop&blast_rank=1&RID=JXJK73J3013